| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Thermosipho africanus | GCCGAAGCACTCCAAAAGG | CGCACCAACATAATCAGCACC | 59.42 | 60.20 | 289 | 182 |
100.00%
|
100.00%
|
| Primer 2 | Thermosipho africanus | GCCGAAGCACTCCAAAAGG | TCCGCACCAACATAATCAGC | 59.42 | 58.62 | 291 | 176 |
100.00%
|
100.00%
|
| Primer 3 | Thermosipho africanus | GCCGAAGCACTCCAAAAGG | CCGCACCAACATAATCAGCAC | 59.42 | 60.20 | 290 | 169 |
100.00%
|
100.00%
|
| Primer 4 | Thermosipho africanus | GCCGAAGCACTCCAAAAGG | TCAGCCTTTTCACCAGTTGC | 59.42 | 58.97 | 252 | 168 |
100.00%
|
100.00%
|
| Primer 5 | Thermosipho africanus | GCCGAAGCACTCCAAAAGG | TTCAGCCTTTTCACCAGTTGC | 59.42 | 59.59 | 253 | 168 |
100.00%
|
100.00%
|
| Primer 6 | Thermosipho africanus | AGTGTTGCAGGAACATCATGT | CCAAATCTTGCCGTTTGTGC | 58.13 | 58.86 | 176 | 148 |
100.00%
|
100.00%
|
| Primer 7 | Thermosipho africanus | AGTGTTGCAGGAACATCATGT | ACCAAATCTTGCCGTTTGTGC | 58.13 | 60.54 | 177 | 148 |
100.00%
|
100.00%
|
| Primer 8 | Thermosipho africanus | AGTGTTGCAGGAACATCATGT | CAAATCTTGCCGTTTGTGCAG | 58.13 | 59.21 | 175 | 148 |
100.00%
|
100.00%
|
| Primer 9 | Thermosipho africanus | GATTTGCAGGTGGAGCTGC | CCGCTTCCATGAAAACCTGG | 59.49 | 59.47 | 274 | 146 |
100.00%
|
100.00%
|
| Primer 10 | Thermosipho africanus | ATTTGCAGGTGGAGCTGC | CCGCTTCCATGAAAACCTGG | 58.31 | 59.47 | 273 | 146 |
100.00%
|
100.00%
|